![]() Then, the plates were incubated at 37 ☌ overnight. From those cultures, 10 µL was collected and inoculated on sterile BHI plates in the form of circular spots. ![]() For this, Streptococcus agalactiae strains were inoculated in 4 mL of BHI broth and incubated at 37 ☌ overnight. The inhibition assays were performed following the spot-on-lawn method, as previously described in. ĭeferred growth inhibition assays were performed to test the 54 Streptococcus agalactiae strains for their ability to produce antimicrobial substances. Bacteriocins are a class of ribosomally synthesized antimicrobial peptides produced by bacteria and are biologically active against other Gram-positive and Gram-negative bacteria. However, bacterial antimicrobial peptides are not to be ignored, especially bacteriocins, since they provide a vast diversity of substances with targeted action. It is worth mentioning that most of the antimicrobial peptides under clinical trials are of human origin due to their expected low toxicity. SOLAR WALK RECA BUSCI TRIALAmong the 36 antimicrobial peptides under clinical trial in 2019, 5 were of bacterial origin. Nonetheless, many antimicrobial peptides are currently under different stages of clinical trials and are expected to be applied in healthcare in the future. The last antimicrobial peptide approved for use was daptomycin, isolated from soil bacteria Streptomyces roseosporus in 1986. Antimicrobial peptides are small molecules of proteinaceous nature produced by all organisms that help protect them from infections by microorganisms they may encounter. One approach to solving this crisis involves using antimicrobial peptides. There are many alternative methods available and under consideration for solving this problem. Thus, the current study shows promising antimicrobial and biotechnological potential for the Streptococcus agalactiae strains. Finally, comparative genomics evidenced high similarity among the genomes, with more significant similarity between the LGMAI_St_11 and LGMAI_St_14 strains. Assessments for secondary metabolites and antimicrobial peptides detected the bacteriocin zoocin A. This gene is associated with resistance against erythromycin, azithromycin, and spiramycin. ![]() Moreover, the mreA gene was identified in the three strains. Virulence potential and antibiotic resistance assessments showed that strain LGMAI_St_08 is slightly more pathogenic than the others. Three genomes were successfully sequenced, allowing for their taxonomic confirmation via a multilocus sequence analysis (MLSA). Deferred plate antagonism assays revealed an inhibition spectrum focused on species of the genus Streptococcus-namely, S. Herein, we assessed the antimicrobial activity and biotechnological potential of 54 Streptococcus agalactiae strains isolated from bovine mastitis. In this scenario, antimicrobial peptides such as bacteriocins represent an alternative solution to traditional antibiotics because they are produced by many organisms and can inhibit bacteria, fungi, and/or viruses. We are now facing a post-antibiotic era in which microbial infections, currently treatable, could become fatal. Antibiotic resistance is one of the biggest health challenges of our time. ![]()
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